Abstract: Objective This study aimed to assess image contrast and evaluate 3.0T T1-weighted magnetic resonance imaging (MRI) sequences for enhanced thoracic spine imaging. Methods Fifty patients with clinically suspected thoracic vertebral disease who underwent thoracic MRI between February 2023 and October 2023 were prospectively included. Each patient underwent MRI using four distinct sequences: Vibe-Dixon, Fl2d-fs, Tse-Dixon, and Tse-fs. A senior MR technician measured the signal intensity (SI) and standard deviation (SD) of the T8 vertebrae and the spinal cord, soft tissue, and air across the four imaging modalities. Signal-to-noise (SNR) and contrast-to-noise ratios (CNR) were calculated. Two experienced radiologists used a double-blind method to score and evaluate the overall image quality, image motion artifacts, and diagnostic confidence of the four imaging sequences, and kappa analysis was performed to assess inter-observer agreement. Results In terms of objective scoring, the SNR values for the Vibe-Dixon sequence in the vertebral body, spinal cord, and soft tissue were higher than those of the Fl2d-fs, Tse-fs, and Tse-Dixon sequences, and the differences were statistically significant. SNR value comparisons between the Vibe-Dixon and Fl2d-fs sequences for soft tissue showed no statistically significant differences, and comparisons between the Tse-fs and Tse-Dixon sequences in the vertebral body, spinal cord, and soft tissue showed no statistically significant differences, as well. The spinal cord soft tissue CNR of the Vibe-Dixon sequence was significantly higher than that of the Tse-Dixon and Tse-fs sequences. Notably, the CNR of the spinal soft tissue with the Vibe-Dixon sequence was significantly lower than that of the Fl2d-fs sequence. In the subjective evaluation, the overall image quality, motion artifacts, and diagnostic confidence of the Vibe-Dixon sequence were superior to those of the Fl2d-fs, Tse-Dixon, and Tse-fs sequences, and these differences were statistically significant. The evaluation of the four sets of images by two radiologists showed good consistency (Kappa=0.72−0.921). Conclusion Compared with the Tse-Dixon, Fl2d-fs, and Tse-fs sequences, the Vibe-Dixon sequences can reduce motion artifacts and obtain better image quality of the thoracic spine-enhanced transection position. In addition, it is feasible to replace Tse and other sequences after enhancement.