Abstract: Objective: We explored the impact of our “Imaging Technology Research Methods” course on cultivation of Comprehensive Research Competence among medical imaging technology undergraduates. Methods: Medical imaging technology undergraduates working in the Department of Radiology, West China Hospital, were sorted into experimental and control groups based on their participation in the “Imaging Technology Research Methods” course. Differences in comprehensive research competence (research thinking, research ability, competency transfer, research related professional competence, and research output) were compared between the two groups. Subgroups in each group comprising students who did or did not pursue postgraduate studies after graduation were also assessed for differences in comprehensive research competence variables. Results: The control and experimental groups respectively comprised 23 and 27 participants. In whole group comparisons, the experimental group significantly outperformed the control group in comprehensive research competence (research thinking, research ability, competency transfer, professional competence-research-related dimension, and research output) (P < 0.05). Subgroup analysis revealed that among students pursuing postgraduate studies after graduation, the experimental group achieved significantly higher scores across all evaluated dimensions (P < 0.05). Among students who did not pursue postgraduate studies, no statistically significant difference in research output was observed between the two subgroups (P > 0.05). Conclusion: The “Imaging Technology Research Methods” course serves as a core pathway that fosters research competency among undergraduates, particularly for those pursuing postgraduate education.