sFRP1过表达对人胃SGC-7901细胞的影响及其影像学研究

林慧敏; 张欢; 潘自来; 瞿颖; 李建芳; 刘欢欢; 诗涔; 严福华

doi:10.15953/j.1004-4140.2015.24.01.01

sFRP1过表达对人胃SGC-7901细胞的影响及其影像学研究

1. 上海交通大学医学院附属瑞金医院放射科, 上海 200025;
2. 上海交通大学医学院附属瑞金医院上海消化外科研究所, 上海 200025

基金项目:

上海交通大学医工交叉基金(YG2012MS48、YG2011MS49)

国家自然基金(81272746)

上海市科委医学引导项目(134119a5900)

详细信息

作者简介:
林慧敏(1990-),女,上海交通大学医学院附属瑞金医院放射科影像医学与核医学专业在读硕士研究生,主要从事研究胃癌影像学表现,Tel:15216653578;E-mail:317169993@qq.com;张欢(1970-),女,上海交通大学医学院附属瑞金医院放射科主任医师,主要从事心血管与胃肠道影像学方面的研究,Tel:13636316478,E-mail:huanzhangy@126.com

通讯作者:
张欢(1970-),女,上海交通大学医学院附属瑞金医院放射科主任医师,主要从事心血管与胃肠道影像学方面的研究,Tel:13636316478,E-mail:huanzhangy@126.com

中图分类号: R445
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出版历程
- 收稿日期: 2014-11-22
- 网络出版日期: 2022-12-05

Effect of sFRP1 Over-expression on Gastric Cancer Cell Line SGC-7901 and Gastric Cancer Imaging

1. Department of Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China;
2. Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

摘要

摘要: 目的:拟探讨分泌型卷曲相关蛋白1(sFRP1)对胃癌SGC-790l细胞及肿瘤影像表现的影响。方法:sFRP1真核表达载体及空载体转染SGC-7901细胞,筛选稳定表达细胞。MTT法检测转染前后细胞增殖变化并绘制细胞增殖曲线。体内实验通过裸鼠腹腔及尾静脉注射建立两组腹腔种植及肺转移肿瘤模型,经PET/CT及能谱CT观察影像学变化。最后通过免疫组织化学、末端脱氧核苷酸转移酶介导的dUTP缺口末端标记实验(TUNEL)进一步明确肿瘤凋亡、增殖、微血管增生情况。结果:体内外实验证实转染后,细胞增殖和成瘤能力增强。PET/CT及宝石CT均出现阳性表现。免疫组化及TUNEL示转染后凋亡减少,增殖增多,微血管增加。结论:sFRP1高表达在体内体外均可促进SGC-790l增殖,具有肿瘤促进作用,有利于在PET/CT及宝石CT获得阳性表现。
- 分泌型卷曲相关蛋白1 /
- SGC-7901 /
- PET/CT /
- 能谱CT
Abstract: Objective: To investigate the effect of sFRP1-overexpression on gastric cancer cell line SGC-7901 and gastric cancer imaging. Methods: sFRP1 expression vector and empty vector were transfected into SGC-7901 cells to obtain stable clones(SGC-7901/sFRP1 and SGC-7901/vector). MTT assays were exploited for cell proliferation(in vitro). Peritoneal implantation and lung metastasis models were established by injecting in peritoneal cavity or caudal vein respectively(in vivo), respectively. PET/CT and spectral CT were utilized for tumor imaging. Then all tumors were dissected and conducted with TUNEL and Immunohistochemistry demonstrating apoptosis, proliferation and MVD. Results: TUNEL and Immunohistochemistry showed that SGC-7901/ sFRP1 had higher relative growth rate, less apoptosis, more proliferation and MVD than that of SGC-7901/vector. Tumors induced with SGC-7901/ sFRP1 showed positive results in PET/CT and spectral CT. Conclusion: SFRP1 up-regulation leads to tumorigenesis in vitro and vivo, contributing to the positive performance in PET/CT and spectral CT.
- sFRP1 /
- SGC-7901 /
- PET/CT /
- spectral CT