Abstract: The Sampula cemetery is located in 14 kilometers southwest of Luopu county, Khotan, Xinjiang province, which is on the southern route of the ancient Silk Road. In the 1980 s, archaeologists carried out several excavations at Sampula cemetery, which a total of 1,369 glass beads were found. Based on the X-ray Computed tomography, this paper analyzes the structure of the four typical eye beads, so as to infer the production process of the eye beads under the background of the times.