Abstract: Objective: To investigate the diagnostic value of enhanced CT combined with serum human mammaryglobin (hMAM) level in detection of axillary lymph node metastasis of breast cancer. Methods: 100 patients with breast cancer treated in our hospital from June 2017 to June 2020 were selected. All patients underwent enhanced CT before operation, and the serum hMAM level was detected. We further analyzed the diagnostic value of enhanced CT combined with serum hMAM level in axillary lymph node metastasis of breast cancer by comparing the consistency between the results of the detection rate of axillary lymph node metastasis obtained respectively by enhanced CT, serum hMAM level, combined examination and the postoperative pathological results which was taken as the standard. Results: 51 patients (51%) were diagnosed as positive axillary lymph node metastasis by serum hMAM and pathological results while 12 patients (12%) with negative axillary lymph node metastasis were diagnosed by both serum hMAM and pathological results, with the consistency rate of 81.5%. Kappa test showed great consistency, 55 cases (55%) were diagnosed as positive axillary lymph node metastasis by enhanced CT and pathological results while 13 cases (13%) with negative axillary lymph node metastasis were diagnosed by enhanced CT and pathology, with the consistency rate of 82.3%. Kappa test showed great consistency, There were 61 cases (61%) with positive axillary lymph node metastasis diagnosed by enhanced CT combined with serum hMAM examination and pathological results while 16 cases (16%) with negative axillary lymph node metastasis diagnosed by enhanced CT combined with serum hMAM examination and pathological results, with the consistency rate of 90.4%. Kappa test showed great consistency, The sensitivity of enhanced CT and serum hMAM were respectively 78.0% and 76.5% while the specificity were respectively 69.5% and 70.3%, which showed no significant difference. The sensitivity and specificity of enhanced CT combined with serum hMAM in detecting lymph node metastasis were significantly higher than those of the two alone. Conclusion: The method of enhanced CT combined with serum hMAM level hold high diagnostic value for axillary lymph node metastasis of breast cancer, and show high consistency with the pathological results. Preoperative comprehensive examination and comprehensive analysis of breast cancer are conducive to the diagnosis of the disease and can provide basis for the selection of surgical methods.